Rapid and differential detection of two analogous enterotoxins of<i>Vibrio cholerae</i>and enterotoxigenic<i>Escherichia coli</i>by a modified enzyme-linked immunosorbent assay
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説明
The principle of a novel ELISA (nylon-slip immuno-test, NSIT) was applied to the differential detection of two analogous enterotoxins, cholera toxin (CT) of Vibrio cholerae and heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli. The results obtained for CT and LT detection by a single test were sufficiently sensitive (87.9 and 100%) and specific (100 and 94.7%) in the differential detection test, when compared with the result of a colony hybridization test with DNA probes. The results suggest that the novel ELISA is applicable to the diagnosis of bacterial infections, by means of differential immunological detection of toxins in a single test.
収録刊行物
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- FEMS Immunology & Medical Microbiology
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FEMS Immunology & Medical Microbiology 17 21-25, 1997-01-01
Oxford University Press (OUP)