説明
iPS cells are attractive for the regenerative medicine. The creation of pluripotent cells from somatic cells has great potential for basic and clinical research and application. Retroviral transduction of four or three transfection factors has been shown to initiate a reprogramming process. Here, we describe the effect of transcription factors regarding the growth and differentiation of mouse iPS cells in normoxia or hypoxia. Furthermore, we introduce the function of hypoxia-inducible factors (HIFs) in mouse iPS cells in hypoxia using RT-PCR and western blotting together with HIFs knockdown techniques.
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キーワード
- Blotting, Western
- Induced Pluripotent Stem Cells
- Cell Culture Techniques
- Cellular Reprogramming
- Hypoxia-Inducible Factor 1, alpha Subunit
- Real-Time Polymerase Chain Reaction
- Transfection
- Cell Hypoxia
- Oxygen
- Repressor Proteins
- Mice
- Gene Expression Regulation
- Basic Helix-Loop-Helix Transcription Factors
- Animals
- Cellular Reprogramming Techniques
- RNA Interference
- RNA, Small Interfering
- Apoptosis Regulatory Proteins
- Cells, Cultured
- Transcription Factors