Separation of inorganic pyrophosphatase from alkaline phosphatase in rabbit dental pulp

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Abstract 1. 1. Inorganic pyrophosphatase (Ppi) in rabbit dental pulp was mainly present in the high speed supernatant fraction. 2. 2. Incisors had the higher PPi activity than molars had, and the highest enzyme activity was found in the apical portion of incisors. 3. 3. PPi was separated into two peaks on DEAE-cellulose column chromatography. One of them was free from the contamination of alkaline phosphatase. Properties of this enzyme were also examined.

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