Development and characterization of polymorphic microsatellite markers for <i><scp>N</scp>eolitsea sericea</i> using <scp>I</scp>llumina paired‐end draft sequencing data

<jats:title>Abstract</jats:title><jats:p>Simple sequence repeat (<jats:styled-content style="fixed-case">SSR</jats:styled-content>) markers were developed and characterized for <jats:italic><jats:styled-content style="fixed-case">N</jats:styled-content>eolitsea sericea</jats:italic> (<jats:styled-content style="fixed-case">B</jats:styled-content>l.) <jats:styled-content style="fixed-case">K</jats:styled-content>oidz. (<jats:styled-content style="fixed-case">L</jats:styled-content>auraceae). Out of 196 designed primer pairs, a total of 144 pairs showed amplification, of which 44 had clear and stable chromatograms. Polymorphism of these 44 loci was tested using 32 individuals sampled from a single population of <jats:italic><jats:styled-content style="fixed-case">N</jats:styled-content>. sericea</jats:italic>. The number of alleles and the polymorphism information content varied from 3 to 12 and 0.271 to 0.853, respectively. A significant departure from the <jats:styled-content style="fixed-case">H</jats:styled-content>ardy‐<jats:styled-content style="fixed-case">W</jats:styled-content>einberg equilibrium was observed in one of the 44 loci. These <jats:styled-content style="fixed-case">SSR</jats:styled-content> markers are useful for population genetic studies and parentage analysis in <jats:italic><jats:styled-content style="fixed-case">N</jats:styled-content>. sericea</jats:italic>, which is one of the most common evergreen species in coastal <jats:italic><jats:styled-content style="fixed-case">P</jats:styled-content>inus thunbergii</jats:italic> forests in central‐western <jats:styled-content style="fixed-case">J</jats:styled-content>apan.</jats:p>