Studies on the Metabolism of D-amino acid in Microorganisms

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  • Part. I. Degradation of D-glutamic acid in Genus Aerobacter

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Several strains of bacteria belonging to genus Aerobacter were found to oxidize D-glutamate rapidly, while tbey show feeble oxidative activity toward the L-form even when they were grown in the medium containing DL-glutamate.The isolation of L-glutamate, a natural amino acid, from its DL-form was achieved by the degradation of D-glutamic acid using one of these strains.This may be the first observation on a natural amino acid obtained from the racemic one by the metabolic action of the organism.A new enzyme, D-glutamic acid oxidase, which is responsible for D-glutamate degradation in this organism and differs from Krebs’ D-amino acid oxidase, has been postulated.

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