Induction of TNF-α mRNA in murine macrophages by virulent and avirulent strains ofSalmonella choleraesuisserovar Typhimurium and serovar Choleraesuis
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説明
TNF-alpha mRNA induction in murine macrophages by virulent and avirulent Salmonella strains was measured in vitro by RT-PCR method. Virulence plasmid-cured strains of S. choleraesuis serovar Typhimurium and serovar Choleraesuis, and rpoS-defective mutant of S. choleraesuis serovar Typhimurium induced significantly higher level of TNF-alpha mRNA than their parent (virulent) strains in macrophages of C3H/HeN mice. When macrophages of LPS-low responder (C3H/HeJ) mice were used, the difference of the induction level was not observed, indicating that LPS was involved in the enhanced level of TNF-alpha mRNA induction by avirulent Salmonella strains. LPSs from virulent and avirulent strains were analysed, but no difference was found for cytokine-inducing activity, and chemical properties. Those results suggested that avirulent Salmonella strains were damaged more easily, and released more LPS in macrophages to enhance TNF-alpha induction.
収録刊行物
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- Microbial Pathogenesis
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Microbial Pathogenesis 22 59-66, 1997-01-01
Elsevier BV
