Radioactive cesium and potassium levels in human placenta: After the Fukushima nuclear power plant accident

s / Placenta 35 (2014) A1eA23 A22 risk of stillbirth at GWNwas defined as a number of womenwith stillbirth at GW. N divided by number of women giving birth at GW N. Results: A total of 32 (29.4%) women experienced stillbirth at median GW of 31 (range, 24±38). Preterm birth (GW37) occurred in 52 (67.5%) of the 77 live born infants. Only 25 (22.9%) women had full-term (GW37) live-born infants. The prospective risk of stillbirth was 29.4% (32/109), 27.5% (25/91), 20.9% (14/67) and 13.0% (6/46) for womenwho reached GW 24, 28, 32, and 36, respectively. Conclusions: As womenwith PMD faces at an extraordinary higher risk of IUFD, early admission to the hospital and intensive monitoring of foetal status should be considered, although whether this policy improves outcome has not been validated. O-097. RADIOACTIVE CESIUM AND POTASSIUM LEVELS IN HUMAN PLACENTA: AFTER THE FUKUSHIMA NUCLEAR POWER PLANT ACCIDENT Makoto Kawamur , Hiroshi Anbe , Fujimori Keiya . a Fukushima Medical University, Department of Obstetrics and Gynecology, Japan; Minamisoma City General Hospital, Department of Obstetrics and Gynecology, Japan Background: The Great East Japan earthquake (followed by large-scale tsunamis) struck Northeastern Pacific Coast of Japan on Mar 11, 2011. It caused TEPCO's Fukushima Daiichi Nuclear Power Plant (NPP) accident, accompanied by radioactive pollution in Fukushima prefecture. Minamisoma City General Hospital, just 23km away from the NPP, restarted the Obstetric services in April 2012. We examined the placental radioactive Cesium and Potassium levels. Methods: We measured the radioactive contents of the 40samples of placenta after the delivery, using high-purity Germanium detectors for Gamma-ray spectrometry (at National Institute of Public Health, Wako, Saitama). All the parturients underwent the Whole-Body Counting (WBC) test of radioactivity. Results: The radioactive content levels are as follows. 134Cs: 0.36Bq/kg, 137Cs: 0.58Bq/kg, total-Cs: 0.94Bq/kg, 40K (natural radioactive): 53.1Bq/kg. Radioactive Cs (from the NPP accident) levels were about 1/50 of the natural radioactive, and much lower than the '87 Italian data, 2years after the Chernobyl NPP accident (total-Cs:11.5Bq/kg). All of the parturients were negative on the WBC test (with cut-off value of 250Bq/body). Conclusion: The Cs levels were much lower than natural radioactive (40K) level, which suggests the efforts to reduce external/internal radioactive exposure in the area has been successful. O-102. DENTAL INFECTION OF PORPHYROMONAS GINGIVALIS INDUCES PRETERM BIRTH Mutsumi Miyauchi , Hisako Furusho , Atsuhiro Nagasaki , Satoshi Urabe , Haruhisa Konishi , Hiroshi Miyoshi , Yoshiki Kudo , Takashi Takata . Department of Oral and Maxillofacial Pathobiology, Institute of Biomedical and Health Sciences, Hiroshima University, Japan; Department of Obstetrics and Gynecology, Hiroshima University, Japan Objectives: Epidemiological studies revealed a link between periodontal disease and preterm delivery, however, the mechanism of the link remains unclear. The present study was aimed to examine the influence of Porphyromonas gingivalis (Pg): one of main periodontal pathogens) on preterm birth with a Pg dental infection mouse model and in vitro analyses with trophoblasts and related cells Methods: In vivo experiment: 8-wksold\C57Bl/6J mice were infected with Pg-w83-strain (108 CFU) from the pulp chamber (Pg group). Mating was started at 6-wks post-infection when a periapical granuloma was established. Gestational day (gd) and birth weight were examined.; At gd15, placental tissues were harvested for IHC staining and PCR. Serum, placental tissue and amniotic fluid were collected for ELISA of cytokines. Uninfected mice were used as negative control (NC). In vitro experiment: HTR-8 (trophoblast), HuhT1 (endothelial cell) and THP1 (monocyte) cell lines were used to assess the effects of Pg and Pg-LPS. Results: Pg group showed 2 days of preterm birth (p<0.01) and low birth weight (p<0.01). The amnion-epithelium was damaged in placenta of Pg group and trophoblasts and endothelial cells became degenerative. Moreover, galectin-3 (Gal-3: an immune regulator) suffused the whole placental tissue and dramatically increased in amniotic fluid (P<0.01). The weaker immuno-expression of CD-31 indicated endothelial damage and immuno-expression of COX-2 and TNF-alpha increased in placental tissue of Pg group. Pg was detected in placental tissue. Pg-LPS up-regulated the expression of COX-2, TNF-alpha and Gal-3. Moreover, recombinant Gal-3 stimulation further increased expressions of them. Conclusion: Dentally infected Pg reaches placental tissue through blood circulation and induces preterm birth via upregulation of COX-2, TNF-a and Gal-3 expression in placental tissue. O-103. EXPRESSION OF TOLL-LIKE RECEPTORS AND PROINFLAMMATORY CYTOKINES IN HUMAN PLACENTA Hiroaki Moroi, Tomomi Kotani, Yukio Mano, Hiroyuki Tsuda, Seiji Sumigama, Fumitaka Kikkawa. Department of Obstetrics and Gyne ...