Plantlet regeneration and propagation from ginger callus

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Abstract Shoot tips of ginger ( Zingiber officinale Rosc.) were used to induce callus on a medium consisting of Murashige and Skoog major elements, Ringe and Nitsch minor elements and organic addenda, 2% sucrose, 0.8% agar and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA). The highest degree of callus formation was obtained from the shoot tips cultured on the medium supplemented with 0.5 mg l −1 2,4-D in combination with 1 mg 1 − BA. Soft calli were separated and then proliferated on the above-mentioned medium. The effect of BA on the regeneration of plantlets from calli was tested. Shoots were successfully regenerated on the medium with BA concentrations of 1 and 3 mg l −1 . Shoot proliferation of the regenerated shoots was induced with the addition of 1-naphthalene acetic acid (NAA) and BA. The shoot-forming capacity of the regenerated shoots was found to decrease on the third subculturing. Root formation was observed regardless of the type of culture medium. Regenerated shoots were successfully acclimatized on a vermiculite medium and ready for field cultivation.

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