Inhibition of Macrophage DNA Synthesis by Immunomodulators

DOI PubMed オープンアクセス

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タイトル別名
  • I. Suppression of [<sup>3</sup>H]Thymidine Incorporation into Macrophages by MDP and LPS

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<jats:title>Abstract</jats:title><jats:p>Oil‐induced guinea pig peritoneal exudate macrophages were found to incorporate actively [<jats:sup>3</jats:sup>H]thymidine without any tissue fluids such as conditioned medium, lymphokines or inflammatory tissue exudates. The [<jats:sup>3</jats:sup>H]thymidine incorporation was markedly suppressed by macrophage stimulants such as muramyl dipeptide (MDP) or bacterial lipopolysaccharide (LPS), while glucosamine incorporation was simultaneously increased by these stimulants. The degree of suppression of thymidine incorporation depended on the cell density, the concentrations of the stimulants, and sera or culture media used. The exposure of macrophages to MDP for 30 min was sufficient to cause significant suppression.</jats:p>

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