Preparation of O2-evolving Photosystem-II subchloroplasts from spinach
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説明
Abstract An O 2 -evolving Photosystem II subchloroplast preparation was obtained from spinach chloroplasts, using low concentrations of digitonin and Triton X-100. The preparation showed an O 2 evolution activity equivalent to 20% of the uncoupled rate of fresh broken chloroplasts, but had no significant Photosystem-I-dependent O 2 uptake activity. The preparation showed a chlorophyll a b ratio of 1.9 and a P-700 chlorophyll ratio of 1 2400 . Absorption spectra at room temperature and fluorescence emission spectra of chlorophyll at 77 K suggested a significant decrease in Photosystem I antenna chlorophylls in the O 2 -evolving Photosystem II preparation.
収録刊行物
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- Biochimica et Biophysica Acta (BBA) - Bioenergetics
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Biochimica et Biophysica Acta (BBA) - Bioenergetics 679 347-350, 1982-02-01
Elsevier BV