Suppression of<i>α</i>Smooth Muscle Actin Expression by IFN-<i>γ</i>in Established Myofibroblast Cell Lines
書誌事項
- 公開日
- 2007-10-01
- DOI
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- 10.1089/jir.2007.0028
- 公開者
- Mary Ann Liebert Inc
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説明
The purpose of this study is to investigate the effect of interferon-gamma (IFN-gamma) on myofibroblast generation using four myofibroblast cell lines that we established from Dupuytren's nodules. Cells were cultured in Earl's medium with 15% fetal bovine serum (FBS) and plated on a microplate at a density of 10(4) cells/well. These cells were treated with various concentrations of IFN-gamma (0-1000 U/mL) for 3 days. By cell-capture enzyme immunoassay (CC-EIA), immunofluorescence staining, and reverse transcriptase polymerase chain reaction (RT-PCR) analysis, we evaluated the effect of IFN-gamma on the expression of alpha smooth muscle actin (alphaSMA) in these cells. CC-EIA and RT-PCR analysis showed that IFN-gamma suppressed alphaSMA expression, and immunofluorescence microscopy demonstrated that IFN-gamma markedly decreased the number of alphaSMA-positive cells. These results showed that IFN-gamma strongly inhibits myofibroblast generation in established myofibroblast cell lines and suggest the possibility of using IFN-gamma therapy for contractile disease.
収録刊行物
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- Journal of Interferon & Cytokine Research
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Journal of Interferon & Cytokine Research 27 835-840, 2007-10-01
Mary Ann Liebert Inc