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Abstract 1. The effect of monovalent cations on aminoacyl-tRNA formation in the absence of added Mg2+ has been studied. 2. In valyl-tRNA formation, all monovalent cations studied (NH+4 Li+, Na+ and K+) are stimulatory, with NH+4 and Li+ being the most effective. An optimum concentration of monovalent cations is between 100 and 200 mM. 3. In leucyl-tRNA formation, NH+4, Li+ or K+ stimulates the reaction significantly. Not only the yield of leucyl-tRNA, but also the rate of the reaction is almost the same as those observed in the presence of an optimal amount of Mg2+. 4. In isoleucyl-tRNA formation, only NH+4 is stimulatory. 5. A synergistic effect of Mg2+ and some monovalent cations is observed in valyl-, isolecyul- and phenylalanyl-tRNA formation. 6. In the presence of NH+4, K+ or Na+, PPi-ATP exchange and amino acid hydroxamate formation are not observed, while they do occur in the presence of Li+. 7. It is assumed that in some aminoacyl-tRNA formations certain monovalent cations can replace Mg2+ either completely or partially.
収録刊行物
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- Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 238 314-323, 1971-05-01
Elsevier BV
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キーワード
- Chromatography, Paper
- Phenylalanine
- Acetates
- Lithium
- Sodium Chloride
- Hydroxamic Acids
- Hydroxylamines
- Ammonium Chloride
- Chromatography, DEAE-Cellulose
- Potassium Chloride
- Ligases
- Adenosine Triphosphate
- Methionine
- Chlorides
- RNA, Transfer
- Leucine
- Escherichia coli
- Histidine
- Magnesium
- Isoleucine
- Edetic Acid
- Aspartic Acid
- Chromatography
- Lysine
- Tryptophan
- Phosphorus Isotopes
- Valine
- Diphosphates
- Enzyme Activation
- Kinetics
- Tyrosine
- Hydroxyapatites
- Dialysis