An application of the cycloborate formation of steroids to the analysis of urinary neutral steroids by gas-liquid chromatography

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Horning et al [l] developed a method for the simultaneous analysis of urinary steroid metabolites by gas-liquid chromatography (GLC), however, many peaks of metabolites seemed to overlap on the gas chromatogram. To avoid these overlappings, several additional means have been employed; prior separation with thin layer and/or column chromatography [2-41 and analysis by a capillary column-equipped device [5,6]. We have also developed a new pretreatment procedure applying to cycloborate formation of steroids according to their characteristic structures. This paper presents details of the new procedure.

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