A pregnancy case report of alagille syndrome; expression of NOTCH signaling in the placenta

s / Placenta 35 (2014) A1eA23 A6 hyalinized material. Surgical procedures, such as dilatation and curettage and cesarean section, might increase the risk of PSN/P. It has been suggested that PSN/P might be consequence of disturbances to the implantation, resulting in abnormal involution of the placental site. The subjective symptom is menorrhagia or intermenstral bleeding, the effect on fertility is unknown. O-001. A PREGNANCY CASE REPORT OF ALAGILLE SYNDROME; EXPRESSION OF NOTCH SIGNALING IN THE PLACENTA Kazue Togashi , Megumi Sato , Akira Sato , Yurina Kameta , Reiko Takayama , Hiroshi Miura , Yukihiro Terada . Akita University Graduate School of Medicine, Department of Obstetrics and Gynecology, Japan; Akita City Hospital, Japan; Kazuno Kosei Hospital, Japan Background: Pregnancy in patients with Alagille syndrome (AS) is rare. It is characterized by chronic cholestasis resulting from intrahepatic bile duct paucity. 114 Japanese AS patients were reported in 2009. Case report: A 22 year old female with history of a miscarriage 7months previously was seen at a hospital for edema and amenorrhea. She was diagnosed at 27 weeks and 4 days of gestation by fetal biparietal diameter and transferred to our maternity unit. Her physical examination was remarkable for low platelets, hypertension, proteinuria and ascites. Her medical record from pediatrics in our hospital revealed that she had AS; hepatic, cardiac, facial and skeletal anomalies. Anesthetic difficulty was predicted. Her thrombocytopenia was caused by liver cirrhosis and hypersplenism. The patient had no esophageal varices and no intracranial vessel abnormalities. Due to the deterioration of her renal function a cesarean section under general anesthesia was performed at 29 weeks. Atonic bleeding was treated with an intrauterine balloon. Management of acute pulmonary edematous, severe proteinuria and hypertension required several days of observation and treatment. After a couple of months her blood pressure and proteinuria had returned to normal levels; meaning that she had been preeclamptic. Conclusion: An awareness of possible organ involvement and a multidisciplinary approach is required in the management of pregnancy with AS. This fascinating case might hopefully provide perspectives for both future research into treatment of Alagille syndrome and human placentation of pregnancy complicated by preeclampsia. O-002. IDENTIFICATION AND CHARACTERIZATION OF HTR-8/SVNEO SP CELLS. Tetsunori Inagaki , Kiyoko Kato , Soshi Kusunoki , Koichi Tabu , Hitomi Okabe , Izumi Yamada , Tetsuya Taga , Akemi Matsumoto , Toshiaki Takezawa , Shintaro Makino , Satoru Takeda . a Jyuntendo Hospital, Japan; Kyushu University, Japan; c Tokyo medical and dental University, Japan; National Institute of Agrobiological Sciences, Japan Goals: We investigated the side population cells in HTR-8/SVneo to identify the trophoblast stem cell marker, and characterize trophoblast stem cells. Method: We performed microarray expression analysis to search for upregulated genes in trophoblast stem cell using a set of HTR-8/SVneo side population and non-side population cells. The expression of several candidate trophoblast stem cell markers were investigated by real time PCR, and performed knockdown of up-regulated genes expression using siRNA to analyze the association of up-regulated genes expression with trophoblast phenotype. Finally three dimensional cell culture system was established to analyze the association with epithelial-mesenchymal transition. Result: LY6D gene was highly up-regulated in side population cells. LY6D gene reduced its expression in the course of seven days’ cultivation in differentiation medium. Side population cells tended to reduce its fraction by treatment of LY6D siRNA. On ontology analysis, two Gene GO pathways of epithelial-mesenchymal transition were involved. Epithelialmesenchymal transition was induced by the co-existence of endometrial cells and side population cells. Conclusion: This is the first report to have identified the expression of LY6D and demonstrated induction of epithelial-mesenchymal transition in HTR-8/SVneo side population cells. O-004. CLINICAL APPLICATION OF HUMAN AMNION-DERIVED MESENCHYMAL STEM CELLS FOR THE TREATMENT OF ACUTE GVHD Kenichi Yamahara , Akihiko Taguchi , Toshihiro Soma , Hiroyasu Ogawa , Tomoaki Ikeda , Jun Yoshimatsu . Department of Regenerative Medicine, National Cerebral and Cardiovascular Center, Japan; Department of Regenerative Medicine Research, Foundation for Biomedical Research and Innovation, Japan; Division of Hematology, Department of Internal Medicine, Hyogo College of Medicine, Japan; Department of Obstetrics & Gynecology, Mie University Faculty of Medicine, Japan; Division of Maternal Fetal Medicine and Gynecology, National Cerebral and Cardiovascular Center, Japan Purpose: We have demonstrated that, similar to bone marrow-derived mesenchymal stem cells (BM-MSCs), fetal appendage-derived MSCs possess the differe ...