Incorporation and replication of foreign metaphase chromosomes in cultured mammalian cells

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Abstract To examine whether metaphase chromosomes, on incorporation into mammalian cells, were degraded completely or integrated and replicated in the recipient cells, the following experiments were carried out. 1. 1. Metaphase chromosomes isolated from rat ascites cells were added to primary cultures of embryonic mouse cells (2 n = 40). Two and 4 days after the chromosome addition, several chromosomes which were presumed to be of rat origin could be detected in mouse cells. These mouse cells contained 41 chromosomes. The frequency of appearance of rat chromosomes in these cells was 0.9% at 2 days and 0.3% at 4 days. 2. 2. Rat chromosomes were added with 3 H-thymidine to the mouse cells. After 2 days of cultivation, five cells were found which contained a rat chromosome labeled with 3 H-thymidine. These aberrant mouse cells appeared at a frequency of about 1 per 3 to 4 × 10 5 metaphases. 3. 3. Metaphase chromosomes fully labeled with 3 H-thymidine were isolated and were added to the cultured hamster cells. When 2 × 10 6 metaphases were examined 2 days later, only 2 were found which contained a semi-conservatively labeled chromosome in the presence of unlabeled hamster chromosomes.

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