Morphological responses of Betula protoplasts in fiber spinning

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<jats:title>Abstract</jats:title> <jats:p>In a previous article, it was reported that, under stress conditions caused by Ca<jats:sup>2+</jats:sup> ions, isolated protoplasts of <jats:italic>Betula platyphylla</jats:italic> leaves release a bundle of hollow fibrils as a stress-induced response. In the present article, details of this phenomenon have been investigated. As a special method, light microscopy combined with a ‘through focus variation’ technique was applied. In a regular culture medium, protoplasts could regenerate the cell walls, whereas protoplasts submitted to Ca<jats:sup>2+</jats:sup> stress could not. Under stress conditions, the protoplasts inflated and formed a huge vacuole and, after 28 days, secreted the peculiar gigantic fibril with a diameter of 150 μm. The giant fibril is composed of (1→3)-β-glucan chains, i.e., the material of callose tissue.</jats:p>

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  • Holzforschung

    Holzforschung 66 2012-03-01

    Walter de Gruyter GmbH

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