Characterization of translucent segments observed in an<i>smbA</i>mutant of<i>Escherichia coli</i>
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説明
The smbA gene of Escherichia coli is essential for cell proliferation. The smbA2 mutant shows cold-sensitive colony formation at 22 degrees C. A novel morphological phenotype, formation of a translucent segment at midcell or at a cell pole, was observed by phase-contrast microscopy at a high frequency in the smbA2 mutant cells incubated in L medium lacking NaCl at 22 degrees C, but not observed in L medium containing 1% NaCl or 20% sucrose at the same temperature. No translucent segment was observed in the wild-type cells in any of the media used. Electron microscopic observation revealed that the translucent segments resulted from the enlargement of a periplasmic space by separation of the inner membrane from the peptidoglycan layer and the outer membrane.
収録刊行物
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- FEMS Microbiology Letters
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FEMS Microbiology Letters 116 61-66, 1994-02-01
Oxford University Press (OUP)