Xenopus Xenf: an early endodermal nuclear factor that is regulated in a pathway distinct from Sox17 and Mix-related gene pathways
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説明
We report a novel zygotic gene encoding a Xenopus endodermal nuclear factor, Xenf. Expression of Xenf starts at the late blastula stages and is decreased after gastrulation. Xenf shows no structural homology to any known proteins. When GFP-tagged Xenf is overexpressed in Xenopus cells, Xenf protein is localized to the nucleus, associating closely with the chromosomes. In animal cap assays, Xenf expression is strongly activated by mRNA injection of Vg1 and VegT, maternal vegetal genes that can induce endodermal differentiation. In contrast, Xenf is not induced by endoderm-inducing zygotic transcription factors such as Sox17 and Mix-related genes. In turn, Xenf does not activate expression of Sox17, Mixer or Milk. Thus, Xenf is regulated by maternal vegetal positional information in a parallel manner to Sox17 and Mix-related gene pathways.
収録刊行物
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- Mechanisms of Development
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Mechanisms of Development 91 81-89, 2000-03-01
Elsevier BV
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キーワード
- Embryology
- DNA, Complementary
- Zygote
- Ubiquitin-Protein Ligases
- Molecular Sequence Data
- Reptilian Proteins
- Xenopus Proteins
- Xenopus laevis
- Transforming Growth Factor beta
- SOXF Transcription Factors
- Animals
- Tissue Distribution
- Amino Acid Sequence
- RNA, Messenger
- Peptide Synthases
- Glycoproteins
- Homeodomain Proteins
- Base Sequence
- Endoderm
- High Mobility Group Proteins
- Gene Expression Regulation, Developmental
- Nuclear Proteins
- Proteins
- DNA-Binding Proteins
- T-Box Domain Proteins
- Biomarkers
- Developmental Biology
- Subcellular Fractions
- Transcription Factors