A Sensitive and Simple Determination of Human α‐fetoprotein by Enzyme‐electroimmunodiffusion

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<jats:p>A simple and sensitive determination method of α‐fetoprotein was developed by introducing α‐fetoprotein horseradish peroxidase conjugate into electroimmunodiffusion.</jats:p><jats:p>The precipitin line formed was directly visualized by staining for peroxidase activities. The sensitivity was 32 ng/ml. Electrophoretic activities of AFP were analyzed by two dimensional electrophoresis applying the present method to the second electrophoresis.</jats:p><jats:p>AFP from normal adults and several patients was confirmed to migrate as an α‐globulin as AFP of hepatoma, yolk sac tumor and fetuses did.</jats:p><jats:p>AFP‐like activities were detected in β‐γ‐globulin region in some samples but these were not due to AFP‐anti‐AFP interactions.</jats:p>

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