Incorporation of<sup>14</sup>C-Linoleic Acid Hydroperoxides and Their Secondary Products into the Cells of<i>Escherichia coli</i>
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説明
Some differences between linoleic acid hydroperoxides (LAHPO) and their secondary products in their function in cell damage were determined. E. coli cells were incubated with the autooxidized products of linoleic acid-l-14C; hydroperoxides and their secondary products. At 0 hr the secondary products showed a higher tendency to adhere to the cell surface, compared with LAHPO. The distribution of radioactivity in the precipitate and the soluble fractions showed that a major portion of the radioactivity incorporated was contained in the soluble fraction in the case of LAHPO, while in the case of the secondary products the radioactivity was contained mostly in the precipitate fraction. A positive relationship between a lowering of the inhibitory effect and a decrease in the incorporation was observed in the case of LAHPO when antioxidants were added to the reaction system. No such effect was observed with the secondary products.These results may show that the inhibition of growth by LAHPO is due to a radical-...
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 35 1254-1258, 1971-08-01
Oxford University Press (OUP)