Corrigendum to “Transcription‐independent role of Bach1 in mitosis through a nuclear exporter Crm1‐dependent mechanism” [FEBS Letters 586 (2012) 448–454]

Fig. 5. Possible role of Bach1 in regulating the mitotic chromosomes. (A) The effects of endogenous BACH1 depletion on the subcellular localization of the chromokinesin, Kid. After treatment with MG132 for 8 h, cells were stained with an anti-Kid antibody. The arrowhead indicates the spindle localization of Kid. Scale bar, 10 lm. (B) The effects of CLS-deletion on the Bach1 localization during the mitotic phase. Cells treated with MG132 and LMB were stained with an anti-Bach1 antibody. Images were obtained from H2B-GFP-positive cells indicating successive transfection (data not shown). The arrowhead indicates the spindle localization of Bach1. Scale bar, 10 lm. (C) A schematic representation of the domain function in Bach1 in interphase and the mitotic phase. The CLS highly conserved between Bach1 and Bach2 shows similarity to the yeast Yap1 Crm1-binding domain, in which two cysteine residues (asterisk) form disulfide bonds with the internal domain and respond to oxidative stress. Based on their similarity, the Bach1 CLS was modeled on the Yap1 structure (PDB ID: 1SSE). MT indicates microtubules. (D) The possible role of Bach1 in mitotic chromosome regulation during metaphase. Bach1 exclusion from the mitotic chromosome was sensitive to LMB treatment. In contrast, CLS deletion from Bach1 abolished both the landing onto and exclusion from mitotic chromosomes. As a consequence, the chromosome arm alignment is dysregulated in the cells expressing the CLS-deleted Bach1 mutant (DC1).