Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum
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説明
Abstract A new reaction model for transphosphatidylation and hydrolysis reactions by PLD has been constructed and simulated under various experimental conditions. The reaction model is based on the Ping-Pong mechanism and composed of PLD–phosphatidyl complex intermediates. In order to accommodate the inhibition of the initial reaction rate at high nucleophile concentration, a phosphatidyl–enzyme complex associating with two nucleophile molecules [P–PLD–X2] is introduced into the transphosphatidylation scheme of the proposed reaction model. In both cases (phosphatidylethanolamine (PE) production, and phosphatidylglycerol (PG) production from phosphatidylcholine (PC)), the simulation results agreed well with the experimental data. While the PE production rate at the initial state was inhibited at high concentrations of the nucleophile (ethanolamine), the PG production rate did not show inhibition. Combination of the PE and PG production schemes is expected to make multi-phospholipid production from PC possible, thus, making this model of potential use for phospholipid synthesis in engineering applications.
収録刊行物
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- Biochemical Engineering Journal
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Biochemical Engineering Journal 10 115-121, 2002-03-01
Elsevier BV