Kinetic and Thermodynamic Study of Syn−Anti Glycosyl Isomerization in Aqueous Solutions of AMP, ADP, and ATP by Ultrasonic Relaxation Methods
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説明
Ultrasonic absorption coefficients in aqueous solutions of adenosine 5‘-triphosphate (ATP) were measured at 25 °C as a function of concentration and pH in the frequency range from 0.8 to 220 MHz. An ultrasonic relaxational absorption with two relaxation frequencies was observed near pH 3. However, above pH 12, only a single relaxational absorption was detected at around 100 MHz, and this relaxation is the focus of this report. The relaxation frequency is independent of the concentration and pH at around pH 12, and the maximum absorption per wavelength increases linearly with concentration. From these results, the cause of the relaxation was attributed to a syn−anti glycosyl isomerization reaction of ATP. The rate of the rotational motion in ATP was found to be smaller than that for ADP and greater than those for AMP and adenosine. These results are discussed in relation to the charge distribution and the effect of the size of the phosphate group of the nucleotides. To examine the reaction mechanism furthe...
収録刊行物
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- The Journal of Physical Chemistry B
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The Journal of Physical Chemistry B 103 3754-3757, 1999-04-16
American Chemical Society (ACS)