Comparative studies of the enzymes involved in 2,3-bisphosphoglycerate metabolism of rabbit erythrocytes and muscle cells

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1. 1. The enzymes participating in 2,3-bisphosphoglycerate metabolism were purified from erythrocytes and muscle cells of rabbit (Oryctolagus coniculus domesticus) in order to clarify why this compound accumulates in erythrocytes but not in tissue cells. 2. 2. Two irreversible reactions, synthesis and breakdown of 2,3-bisphosphoglycerate, are catalyzed by one multifunctional enzyme in erythrocytes. 3. 3. Muscle cells have a novel enzyme, 2,3-bisphosphoglycerate phosphatase, which has never been found before in mammalian erythrocytes and which may be mainly responsible for keeping this compound at low levels.

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