Characterization of antennary gland Na,K-ATPase in the freshwater crayfish, Procambarus clarki girard

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Abstract 1. 1. Na, K-ATPase activity was demonstrated in a heavy microsomal fraction of antennary gland preparations of the freshwater crayfish, Procambarus clarki . 2. 2. The highest activity was found in 100 mM of sodium and 4mM of potassium in the presence of 1 mM of magnesium at pH 8.5. 3. 3. K m of Na,K-ATPase for ATP was 9.0 × 10 −4 M . 4. 4. A complete inhibitory effect on Na,K-ATPase by ouabain was found with 10 −2 M ouabain. The pl 50 was 4.1. 5. 5. Both renal Na,K-ATPase and Mg-ATPase were inhibited by POMB. The pI 50 was 6.3 and 5.0, respectively. Inhibitory effect of POMB was restored by cysteine. 6. 6. The activity of gill Na,K-ATPase was increased only when the animals were maintained in 50% seawater.

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