Subunit flow in F-actin under steady-state conditions

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We developed a novel method to determine the subunit exchange rates of F-actin at its terminals under quasi-steady-state conditions by using a powerful fluorescent probe, N-(1-pyrenyl)iodoacetamide. The applicability of the method was checked with regard to both theoretical and experimental aspects. We determined the rates of subunit exchange of F-actin and F-actin-tropomyosin complex under various ionic conditions. We found that: (i) KCl accelerated both on and off rates at each end, and lowered the critical concentration of the P-end while the critical concentration of the B-end was not affected; (ii) binding of tropomyosin drastically reduced the subunit flow in F-actin by suppressing the off rate principally of the P-end. It is therefore believed that tropomyosin exerts an anisotropic constraint on F-actin and regulates its dynamic polarity.

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