Inter- and intrasyngenic variation of Paramecium hemoglobin—I. Paramecium aurelia complex

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Abstract 1. 1. Hemoglobins (Hbs) from Paramecium aurelia complex showed a considerable degree of inter- and intrasyngenic variations by polyacrylamide gel electrophoresis (PAGE). 2. 2. All of the Hbs from various stocks of different syngens were resolved into definite 12 variants, which gave expression to a difference in the mobility by PAGE. 3. 3. An argument that the multiplicity of Paramecium Hb may be an artifact caused by endogenous proteases, was not supported by the facts obtained on a crude Hb sample prepared under the presence of antipain, leupeptin, pepstatin and phosphoramidon in sufficient concentrations. 4. 4. The Hb variants can be subdivided into two groups by their molecular weights, taking either value of 11,000 and 13,000.

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