Characterization of the cupin‐type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralisa
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説明
<jats:p>The gene encoding phosphoglucose isomerase was cloned from <jats:italic>Thermococcus litoralis</jats:italic>, and functionally expressed in <jats:italic>Escherichia coli</jats:italic>. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe<jats:sup>2+</jats:sup>, but inhibited by Zn<jats:sup>2+</jats:sup> and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in <jats:italic>k</jats:italic> <jats:sub>cat</jats:sub>. The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.</jats:p>
収録刊行物
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- FEBS Letters
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FEBS Letters 535 200-204, 2003-01-10
Wiley
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キーワード
- Archaeal Proteins
- Iron
- Molecular Sequence Data
- Binding, Competitive
- Catalysis
- Structure-Activity Relationship
- Amino Acid Sequence
- Cloning, Molecular
- Enzyme Inhibitors
- Chelating Agents
- Binding Sites
- Sequence Homology, Amino Acid
- Circular Dichroism
- Glucose-6-Phosphate Isomerase
- Temperature
- Enzyme Activation
- Molecular Weight
- Thermococcus
- Zinc
- Spectrophotometry
- Mutagenesis, Site-Directed
- Electrophoresis, Polyacrylamide Gel
- Dimerization
