A stalk-specific wheat germ agglutinin binding protein, wst34, in <i>Dictyostelium discoideum</i> can be detected with antiserum raised against <i>Dictyostelium mucoroides</i> stalk

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<jats:p> A new stalk-specific wheat germ agglutinin (WGA) binding protein, wst34, has been identified in Dictyostelium discoideum and purified by the use of preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis and a WGA-affinity column. In normal development, wst34 appears during culmination and is maintained in stalk cells. It has a molecular mass of 34 kilodaltons and a pI value of 5.5–6.5. A polyclonal antiserum raised against stalk cell proteins of Dictyostelium mucoroides recognizes wst34 in western blots of D. discoideum proteins.Key words: Dictyostelium discoideum, Dictyostelium mucoroides, wheat germ agglutinin. </jats:p>

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詳細情報 詳細情報について

  • CRID
    1873398393024069376
  • DOI
    10.1139/o90-101
  • ISSN
    12086002
    08298211
  • PubMed
    2222995
  • データソース種別
    • OpenAIRE

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