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Identification of Th2-type suppressor T cells among<i>in vivo</i>expanded ocular T cells in mice with experimental autoimmune uveoretinitis
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Description
<jats:title>SUMMARY</jats:title><jats:p>Experimental autoimmune uveoretinitis (EAU), which is a T cell mediated organ specific autoimmune disease, is induced by immunization with interphotoreceptor retinoid binding protein (IRBP) in susceptible strains of mice. It has been found that IRBP-derived peptide 518–529 (p518–529) generates Th2-type responses and inhibits IRBP-induced EAU, indicating that the p518–529 might be an epitope for suppressor T cells in IRBP-induced EAU. First, we observed that there were T cells producing the Th2 type cytokines such as IL-4 and IL-10 in late phase of EAU. Furthermore, to examine whether p518–529-reactive T cells expand in the eye during EAU, T cell receptor (TCR) of ocular T cells was compared with that of p518–529 reactive T cells in spleen from mice with EAU by PCR-single strand conformation polymorphism (PCR-SSCP) and nucleotide sequence analysis. SSCP and sequence analyses indicated that p518–529 reactive TCR BV10+ T cells bearing amino acid motif(PWG) and TCR BV13+ T cells bearing amino acid motif(PGLGGY) in their complementary-determining region 3 (CDR3) region were clonally expanding in ocular tissues on day 28 after immunization, although these T cells were not detected on day 14. These findings demonstrate that p518–529 reactive Th2-type T cells expand oligoclonally in the uveitic eyes in the late stage of EAU and may function as Th2-type suppressor T cells for improvement of the disease.</jats:p>
Journal
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- Clinical and Experimental Immunology
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Clinical and Experimental Immunology 124 1-8, 2001-04-01
Oxford University Press (OUP)
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Keywords
- DNA, Complementary
- Amino Acid Motifs
- Receptors, Antigen, T-Cell
- Autoantigens
- Polymerase Chain Reaction
- T-Lymphocytes, Regulatory
- Retina
- Nervous System Autoimmune Disease, Experimental
- Uveitis
- Epitopes
- Interferon-gamma
- Mice
- Th2 Cells
- Animals
- RNA, Messenger
- Eye Proteins
- Uvea
- Polymorphism, Single-Stranded Conformational
- Retinitis
- Peptide Fragments
- Clone Cells
- Interleukin-10
- Specific Pathogen-Free Organisms
- Retinol-Binding Proteins
- Female
- Immunization
- Interleukin-4
- Spleen
Details 詳細情報について
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- CRID
- 1873398393030248064
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- ISSN
- 13652249
- 00099104
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- PubMed
- 11359436
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- Data Source
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- OpenAIRE