Rapid separation of barbiturates and benzodiazepines by capillary electrochromatography with 3‐(1,8‐naphthalimido)propyl‐modified silyl silica gel

DOI PubMed

この論文をさがす

説明

<jats:title>Abstract</jats:title><jats:p>A capillary electrochromatographic (CEC) method was applied to the simultaneous separation of barbiturates (barbital, phenobarbital, secobarbital and thiopental) and benzodiazepines (nitrazepam, diazepam and triazolam). The separation was performed in a 75 µm i.d. capillary, packed with 3‐(1,8‐naphthalimido)propyl‐modified silyl silica gel (NAIP), studying the effects of buffer pH and mobile phase composition. Using an applied voltage of 20 kV and the short‐end injection method (9 cm capillary effective length), the mobile phase of 1.0 m<jats:sc>m</jats:sc> citrate buffer (pH 5.0) containing 45% methanol provided the baseline separation of seven toxic drugs in less than 9 min. In CEC with NAIP, the benzodiazepines were separated by the combination of hydrophobic and <jats:italic>π</jats:italic>–<jats:italic>π</jats:italic> interactions, whereas the separation of the barbiturates was based on the hydrophobic interaction. Copyright © 2004 John Wiley & Sons, Ltd.</jats:p>

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1873398393049807232
  • DOI
    10.1002/bmc.346
  • ISSN
    10990801
    02693879
  • PubMed
    15273980
  • データソース種別
    • OpenAIRE

問題の指摘

ページトップへ