Characterization of cyclic AMP and cyclic GMP phosphodiesterases in Tetrahymena cilia

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Abstract 1. 1. The activities of cyclic AMP and cyclic GMP phosphodiesterases were found to be associated with both soluble and particulate fractions in Tetrahymena cilia. 2. 2. The properties of these enzymes in ciliary fractions were similar to those of cell bodies in K m , pH dependency, inhibition by methylxanthine and lack of a requirement for Mg2+. 3. 3. These enzyme activities of cell bodies of behavioral mutants were higher than those of wild type, while the enzyme activities of cilia did not show differences in both wild and mutant cells.

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