Ultrastructural alterations induced by Amphotericin B in the plasma membrane of Epidermophyton floccossum as revealed by freeze-etch electron microscopy

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Abstract Freeze-etch electron microscopic observations demonstrated that Amphotericin B, a potential antifungal polyene induced profound ultrastructural alterations in the plasma membrane of cells of a human pathogenic fungus, Epidermophyton floccosum ; aggregation of membrane-associated particles (85-A) and formation of depressions (or craters) on the inner fracture face. Thin-sectioning electron microscopy showed “vesiculation” of plasma membranes of Amphotericin B-treated Epidemophyton cells. It can be suggested from the preliminary results that the fungal cells which contain ergosterol as the major sterol are highly sensitive to Amphotericin B.

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