- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Search this article
Description
<jats:p>Four isolates of <jats:italic>Entamoeba dispar</jats:italic> identified by their hexokinase and phosphoglucomutase isoenzyme profile and by their failure to react with <jats:italic>Entamoeba histolytica</jats:italic>‐specific monoclonal antibody (4G6) could be grown in either Diamond's BI‐S‐33 medium, newly developed BCSI‐S (Biosate cysteine starch iron‐serum) medium, or casein‐free YI‐S medium in the presence of <jats:italic>Crithidia fasciculata</jats:italic> (ReF‐1:PRR) sterilized by heating 56° C for 30 min and subsequent incubation with 1% hydrogen peroxide for 24 hours at 4° C. After the cultures were maintained for over 50 passages, the amebae were identified as <jats:italic>E. dispar</jats:italic> by isoenzyme analysis, polymerase chain reaction with <jats:italic>E. histolytica</jats:italic>‐ and <jats:italic>E. dispar</jats:italic>‐specific primers, i.e. p11 plus p12 and p13 plus p14, respectively, and by negative reactivity with monoclonal antibody 4G6. The flagellates added to the culture were judged to be metabolically inactive based on the results of nuclear magnetic resonance spectroscopy, electron microscopy, and polarographic analysis. All of these findings suggest that <jats:italic>E. dispar</jats:italic> can grow in vitro with metabolically inactive <jats:italic>C. fasciculata</jats:italic> as a culture associate.</jats:p>
Journal
-
- Journal of Eukaryotic Microbiology
-
Journal of Eukaryotic Microbiology 45 1998-03-01
Wiley