Structural and functional studies on the human hepatic interleukin-6 receptor. Molecular cloning and overexpression in HepG2 cells

DOI PubMed オープンアクセス

書誌事項

公開日
1991-08-01
DOI
  • 10.1042/bj2770659
公開者
Portland Press Ltd.

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説明

<jats:p>cDNAs coding for the human hepatic interleukin-6 receptor (IL-6-R) have been isolated from a library made from poly(A) RNA of dexamethasone-treated human hepatoma cells (HepG2). We found the hepatic IL-6-R to be identical to the one expressed by leucocytes. A polyclonal antiserum was raised in rabbits against the IL-6-R protein expressed in Escherichia coli. Although the entire IL-6-R protein was used for immunization, only antibodies to the cytoplasmic domain of the IL-6-R were obtained. It is demonstrated by affinity cross-linking and subsequent immunoprecipitation with antibodies against the ligand as well as against the receptor that the cloned cDNA codes for the functional IL-6-R on HepG2 cells. When the hepatic IL-6-R cDNA was overexpressed in HepG2 cells, these cells became more sensitive to low concentrations of IL-6 with respect to the induction of gamma-fibrinogen mRNA.</jats:p>

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詳細情報 詳細情報について

  • CRID
    1873679867887019264
  • DOI
    10.1042/bj2770659
  • ISSN
    14708728
    02646021
  • PubMed
    1872801
  • 本文言語コード
    en
  • データソース種別
    • OpenAIRE

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