Paternal antigen-specific inducible regulatory T cells are increased just before the implantation by seminal fluid-priming

CD86 protein whereas the number of CD11c+CD80+ cells was diminished. The changes in costimulatory molecules expression were dependent on male strain and present at protein levelwithout changes in transcription.Ova-specific lymphocytes proliferation was increased after blocking of CD80 but significantly diminished in mated mice after the blocking of CD86 molecules. The blocking of CD80 significantly decreased cytokines production mainly by F4/80+ cells but IFN-gamma, which concentration was increased.Administrationofblockingabsanti-CD40, -CD86 and -CD80 did not influence the number of fetuses, however blocking of CD80 caused an significant increase of IL-12 (p<0,005) and decrease IL-10 (p<0,01) production. Blocking of CD40 increased the number of Treg lymphocytes with diminished expression of FOXP3 in compare to mice administered with isotype control corresponding to used blocking antibodies. IN CONCLUSION: During mouse pregnancy the shaping of costimulatory phenotype at periphery starts as early as 12hour after mating and the clear advantage of CD86 expression is visible in 3.5 day post mating. The costimulatory phenotype is partially dependent of male genotype. Blocking of costimulatorymolecules CD86, CD80 and CD40 does not influence pregnancymaintenance however cytokine production both in response to specific antigen and during pregnancy remains under control of costimulatory potential of CD80 molecule. The frequency of spleen Treg and a stable FOXP3 level depends on availability of CD40. Supported by Polish National Research Center Project number 474/N-COST/2009/0 and 5239/B/P01/2011/40