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<jats:p>A standalone-type beryllium (Be) high-pressure crystallography vessel has been developed. Using a coupler-joint unit and a pressure valve, we could keep the pressure in the vessel constant at 100±1 MPa for more than 24 h without connecting to a pressure-generating apparatus. Diffraction spots of a glucose isomerase (GI) crystal under 100 MPa were collected using the vessel and a rotating copper-anode in-house x-ray generator (0.8 kW). We successfully collected a 2.0 Å resolution data set of a 0.5 mm size GI crystal in an aqueous solution at 100 MPa.</jats:p>
収録刊行物
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- Review of Scientific Instruments
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Review of Scientific Instruments 81 2010-08-01
AIP Publishing