Effect of single base insertion into anticodon loop of frameshift suppressor tRNA

Hikaru Taira, Takahiro Hohsaka, Masaharu Fukushima, Masahiko Sisido

doi:10.1093/nass/1.1.189

Description

Frameshift suppressor tRNAs containing an additional nucleotide at anticodon loop were prepared and their translational activity was evaluated in an E. coli in vitro translation system. The mutated tRNA(CCCG) was chemically aminoacylated with nitrophenylalanine, and added to the in vitro translation system together with a streptavidin mRNA containing CGGG codon. Western blot analysis indicates that the mutated tRNAs can decode the four-base codon, except 32.1C and 33.1G mutants.

Journal

Nucleic Acids Symposium Series

Nucleic Acids Symposium Series 1 189-190, 2001-11-01

Oxford University Press (OUP)

Keywords

Details 詳細情報について

CRID: 1873961342916697984

DOI: 10.1093/nass/1.1.189

ISSN: 17468272; 02613166

PubMed: 12836328

Data Source

OpenAIRE

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