Histamine release and calcium concentrations in rat mast cells are dependent on intracellular ATP: Effects of prostaglandin D2

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When PGD2 (10 microM), was added to rat mast cells, it caused a rapid increase in adenosine 3',5'-cyclic monophosphate (cyclic AMP) and decrease in adenosine 5'-triphosphate (ATP), both of which recovered to their original levels within 2 min. The accumulation of cyclic AMP was maximal at 30 s after challenge with PGD2. The minimum level of ATP was observed at 30 s after addition of PGD2. The initial rise in [Ca2+]i and the histamine release induced by anti-IgE (200 micrograms/ml) were strongly inhibited at 30 s after incubation of the mast cells with PGD2. Removal of glucose from Tyrode-Hepes solution caused a rapid decrease on ATP level in mast cells, and showed strong inhibition on the rise in [Ca2+]i and histamine release induced by anti-IgE. Addition of glucose to the mast cells induced a time-dependent increase in ATP, and the rises in [Ca2+]i and histamine release were closely correlated with the recovery of ATP. These results suggested that the inhibitory mechanism of PGD2 on the initial rise in [Ca2+]i and histamine release induced by anti-IgE was due to the inhibition of ATP-dependent CA(2+)-release from the intracellular Ca(2+)-stores.

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