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Protocols for Gelfoam® Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods
Description
Gelfoam(®)-histocultured whisker follicles from nestin-driven-green fluorescent protein (ND-GFP) mice produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day 4 and continued growing until at least day 12 after which the hair-shaft length was constant. By day 63 in histoculture, the number of ND-GFP hair follicle-associated pluripotent (HAP) stem cells increased significantly and the follicles were intact. Three-dimensional Gelfoam(®) histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.
