Mechanism of immunological cell injury in isolated rat hepatocytes with anti-rat liver plasma membrane antiserum treatment

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Abstract We examined the changes of phospholipase A 2 (PLA 2 ) activity and of reduced glutathione (GSH), Ca 2+ , and lipid peroxide (LPO) contents in isolated rat hepatocytes treated with anti-liver cell plasma membrane antiserum (ALPMA) following the development of immunological cell injury. Increases in PLA 2 activity and Ca 2+ content and a decrease in GSH content in the ALMA-treated hepatocytes occurred with an increase in release of alanine and asparatate transaminases from the cells. After these changes occurred, LPO level increased in the ALPMA-treated hepatocytes. These results suggest that in ALPMA-treated rat hepatocytes, depletion of intracellular GSH, which has a function of cell membrane stabilization, followed by increases in Ca 2+ level and PLA 2 activity primarily contributes to immunological cell injury and that stimulation of intracellular LPO production consequent of increased PLA 2 activity is secondarily contributory to the cell injury.

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