1173 A NEW DOUBLE-EFFECT THERAPEUTIC STRATEGY USING A HISTONE DEACETYLASE INHIBITOR FOR HCV-ASSOCIATED HEPATOCELLULAR CARCINOMA

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gene expression levels were decreased (30–45%), independently of viral genotype. Total DGAT activity was found increased in Huh7.5 cells infected by JFH1 (1.6±0.2), genotype 1b (2.1±0.2) and genotype 3a (1.9±0.1). DGAT activity in Huh7 cells was increased upon HCV infection (JFH1: 1.6±0.03, G1a: 1.54±0.04 and G3a: 1.9±0.04). Quercetin had an inhibitory effect on DGAT activity (1.5±0.1 fold) when cells were infected by genotype 3a and 1a. Quercetin inhibited viral replication in a dose-dependent manner; quercetin 25uM: 31.23%; 50uM: 42.5% and 100uM: 86.41% compared to interferon treatment (100%inhibition). Conclusions: HCV infection increased DGAT-1 gene expression and DGAT activity, improving viral replication. Quercetin decreased DGAT activity and reduced viral replication. DGAT1 protein can arise as a new target for hepatitis C therapy.

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