Glucose Transporter 4 (GLUT4) mRNA Abundance in the Adipose Tissue and Skeletal‐Muscle Tissue of Ovariectomized Rats Treated with 17β‐Estradiol or Progesterone

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<jats:title>Abstract</jats:title><jats:p><jats:bold>Objective:</jats:bold> To investigate the effects of 17β‐estradiol and progesterone on the expression of glucose transporter 4 (GLUT4) in the adipose tissue and skeletal‐muscle tissue of ovariectomized rats.</jats:p><jats:p><jats:bold>Methods:</jats:bold> Female Sprague‐Dawley rats (<jats:italic>n</jats:italic> = 63) received a daily subcutaneous injection of 10 μg, 50 μg, or 250 μg of 17β‐estradiol (Group E) or of 1 mg, 5 mg or 25 mg of progesterone (Group P) for 3 days, 7 days, or 10 days (<jats:italic>n</jats:italic> = 3, at each dose). The expression of GLUT4 mRNA was assessed by performing ribonuclease protection assays.</jats:p><jats:p><jats:bold>Results:</jats:bold> The levels of GLUT4 mRNA in adipose tissue was significantly reduced by treatment with estradiol, 50 μg or 250 μg, relative to findings in control rats (p < 0.01). No such reductions were seen regarding the progesterone treatment. The level of GLUT4 mRNA in skeletal‐muscle tissue did not change, regardless of treatment.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> In ovariectomized rats, higher than physiologic dosages of 17β‐estradiol can suppress the expression of GLUT4 mRNA in adipose tissue.</jats:p>

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