Bubble-Assisted in-Situ Re-Formation of Artificial Bilayer
説明
This paper proposes a technique to reconstitute a lipid bilayer membrane by injecting an air bubble onto the raptured spot. Our group has been studying the artificial bilayer that can host membrane proteins and can be a fundamental to analyze their functions. The only drawback of the lipid bilayer formed at the interface of two water-in-oil droplets is its sustainability, namely, the bilayer often collapses and the droplets merges by the disturbance like self-exerted oscillation or sloshing. To overcome this issue, the newly proposed technique utilizes a bubble at approximately $4\ \mu \mathrm{L}$ in volume. When the bubble is injected and retracted, the new artificial bilayer is reconstituted. The major advantage of this technique is the in-situ reconstitution of the bilayer during the electrical measurement of the membrane proteins. The proposed technique was successfully verified by confirming the emergence of nanopore membrane proteins.
収録刊行物
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- 2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS)
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2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS) 1022-1023, 2020-01-01
IEEE
