書誌事項
- タイトル別名
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- Distinction between generalized and specialized transduction
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説明
We demonstrated that bacteriophages P1 and P22 were able to form various types of hybrids with six out of seven different R plasmids tested. When the same R plasmid was used for isolation, P1-R hybrids usually carried more resistance markers than P22-R. Several genetical observations suggest that the hybrid prophages carried the resistance markers transposed to the phage genomes without loss of essential phage genes. Upon UV-irradiation the prophages produced phage lysates that transduced the relevant resistance markers at high frequencies by lysogenic conversion. The insertion of the resistance markers was even acquired by the P1 or P22 genomes during one-step growth in R+ cells. Some lytically prepared lysates grown on R+ cells contained the hybrids at a frequency of 10-7 to 10-6/plaqueforming unit. Analysis of P1-transductants for resistance markers of the R plasmids revealed in some cases more specialized transductants than generalized transductants. These results strongly indicate that a precise genetic map of an R plasmid can not be established only on the basis of co-transduction frequencies of the resistance markers of the R plasmid.
収録刊行物
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- Molecular and General Genetics MGG
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Molecular and General Genetics MGG 157 131-138, 1977-01-01
Springer Science and Business Media LLC
