Erratum to: “Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12”
書誌事項
- タイトル別名
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- [FEMS Microbiol. Lett. 152 (1997) 183-188]
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The page is published in its entirety overleaf. The Publisher apologizes for any trouble caused by this error. ### 2.1 Materials To obtain mRNA and genomic DNA from T. fermentans WU-C12, a medium containing 5.0% cornsteep liquor, 3.0% olive oil, 0.1% Na2HPO4, 0.1% MgSO4·7H2O and 0.1% (NH4)2SO4 was used. The pH was initially adjusted to 5.5. Cultivations were carried out with reciprocating shaking (120 rpm) in a Sakaguti flask at 30°C for 2 days. Escherichia coli JM109 was used as a host for plasmids pGEM-T Vector System I (Promega) and pBluescript II KS+ (Stratagene) and E. coli NM514 for lambda phage. ### 2.2 Determination of partial amino acid sequences Extracellular lipase I …
収録刊行物
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- FEMS Microbiology Letters
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FEMS Microbiology Letters 154 423-424, 1997-09-15
Oxford University Press (OUP)