Demonstration data underlying the article: Wide Field Light-sheet Microscopy with Lens-axicon Controlled Two-photon Bessel Beam Illumination

Summary<br> This data record provides details of the data supporting the claims of the article: “Wide Field Light-sheet Microscopy with Lens-axicon Controlled Two-photon Bessel Beam Illumination”, which proposes simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent Bessel beams, towards broadening applications of two-photon excitation in light-sheet microscopy. Type of data: custom-built two-photon excitation digital scanned light-sheet microscope possessing the beam full width at half maximum of 600–1000 μm with less than a 4-μm waist when using 10× illumination lens and ~2-3-μm axial resolution Subject of data: cellular dynamics over the whole body of medaka fish Data The data underlying the related article are contained in the zipped folder ‘DataSubmit.zip’, which is openly available as part of this figshare data record. The zipped folder contains a hierarchical sub-folder structure, which is listed briefly below. Further details on the images in each sub-folder are provided in the Word file ‘DataInfo.docx’. 1. FLT4angiography (related to Fig.5A) This folder includes subfolders numbered 1-3. These are biologically independent samples. Each subfolder includes unsigned 16bit tiff-image sequences labeled as Image_z****_c***.tif (z:z-stack number, c:channel number; c001:GFP, c002:Rhodamine). Sizes of images are 1. 1029×4419px 1130 z-seq 2. 1030×3774px 969 z-seq 3. 1030×3812px 530 z-seq 2. FLT4development (related to Fig.7)<br> This folder includes subfolders numbered 1 and 2. These are biologically independent samples. Each subfolder includes unsigned 8bit z-stack tiff images labeled as t****min.tif. The numbers denote time (minites post fertilization; 1. 4755-9305min, 2. 4890-9220min), and the time-lapse interval is 5min. Sizes of images are 1. 512×512px 350 z-seq 2. 512×512px 360 z-seq 3. Caimaging (related to Fig.8)<br> This folder includes unsigned 16bit tiff images labeled as image_t***_z***.tif. (t: time slice number, z: z-stack number). The time-lapse interval is 4sec. Size of images: 512×512px 67 z-seq <br> Corresponding author(s) for this study Takashi Saitou, Department of Molecular Medicine for Pathogenesis, Graduate School of Medicine, Ehime University, Shitsukawa, Toon, 791-0295, Ehime, Japan. E-mail: t-saitou@m.ehime-u.ac.jp. Tel.: +81 89 960 5045. Fax.: +81 89 960 5052. <br> Ethics All experiments were conducted in accordance with the guidelines of the ethics committee for animal experiments of Ehime University.