A randomized clinical trial to compare P. falciparum gametocytaemia and infectivity following blood-stage or mosquito bite induced controlled malaria infection

メタデータ

公開日
2022-03-30
DOI
  • 10.5061/dryad.vq83bk3nq
公開者
Dryad
データ作成者 (e-Rad)
  • Bousema, Teun
  • Alkema, Manon
  • Reuling, Isaie
  • de Jong, Gerdie
  • Lanke, Kjerstin
  • Coffeng, Luc
  • van Gemert, Geert-Jan
  • van de Vegte-Bolmer, Marga
  • de Mast, Quirijn
  • van Crevel, Reinout
  • Ockenhouse, Christian
  • Ivinson, Karen
  • McCarthy, James
  • Sauerwein, Robert
  • Collins, Katharine

説明

For malaria elimination efforts, it is important to better understand parasite transmission to mosquitoes and to develop models to allow early clinical evaluation of transmission-blocking interventions. We previously described a Controlled Human Malaria Infection protocol for induction of gametocytemia in malaria naïve volunteers by mosquito bite (CHMI-trans) (Reuling et al., 2018).  Here, we compared gametocyte production and infectivity in the CHMI-trans model after bites of Plasmodium falciparum (Pf)- infected mosquitoes  to that after intravenous administration of  Pf-infected-erythrocytes.  Volunteers received (sub) curative treatments with gametocyte-permissive piperaquine or sulfadoxine-pyrimethamine. Blood-stage inoculation induced considerably higher gametocyte densities compared to mosquito bitesthat was predicted by PfAP2-G transcripts indicative of gametocyte commitment, and resulted in Pf-positive mosquito infections in 9/12 volunteers versus 0/12 volunteers after mosquito bite inoculation. Current findings firmly establish the CHMI-trans with intravenous administration of asexual parasites as a model for early clinical evaluation of interventions that aim to interrupt Pf-transmission.

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