A randomized clinical trial to compare P. falciparum gametocytaemia and infectivity following blood-stage or mosquito bite induced controlled malaria infection
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- Bousema, Teun
- 作成者
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- Alkema, Manon
- 作成者
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- Reuling, Isaie
- 作成者
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- de Jong, Gerdie
- 作成者
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- Lanke, Kjerstin
- 作成者
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- Coffeng, Luc
- 作成者
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- de Mast, Quirijn
- 作成者
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- Ivinson, Karen
- 作成者
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- McCarthy, James
- 作成者
メタデータ
- 公開日
- 2022-03-30
- DOI
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- 10.5061/dryad.vq83bk3nq
- 公開者
- Dryad
- データ作成者 (e-Rad)
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- Bousema, Teun
- Alkema, Manon
- Reuling, Isaie
- de Jong, Gerdie
- Lanke, Kjerstin
- Coffeng, Luc
- van Gemert, Geert-Jan
- van de Vegte-Bolmer, Marga
- de Mast, Quirijn
- van Crevel, Reinout
- Ockenhouse, Christian
- Ivinson, Karen
- McCarthy, James
- Sauerwein, Robert
- Collins, Katharine
説明
For malaria elimination efforts, it is important to better understand parasite transmission to mosquitoes and to develop models to allow early clinical evaluation of transmission-blocking interventions. We previously described a Controlled Human Malaria Infection protocol for induction of gametocytemia in malaria naïve volunteers by mosquito bite (CHMI-trans) (Reuling et al., 2018). Here, we compared gametocyte production and infectivity in the CHMI-trans model after bites of Plasmodium falciparum (Pf)- infected mosquitoes to that after intravenous administration of Pf-infected-erythrocytes. Volunteers received (sub) curative treatments with gametocyte-permissive piperaquine or sulfadoxine-pyrimethamine. Blood-stage inoculation induced considerably higher gametocyte densities compared to mosquito bitesthat was predicted by PfAP2-G transcripts indicative of gametocyte commitment, and resulted in Pf-positive mosquito infections in 9/12 volunteers versus 0/12 volunteers after mosquito bite inoculation. Current findings firmly establish the CHMI-trans with intravenous administration of asexual parasites as a model for early clinical evaluation of interventions that aim to interrupt Pf-transmission.