Data from: Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription

DOI

メタデータ

公開日
2019-01-01
DOI
  • 10.5061/dryad.c8n0748
公開者
Dryad
データ作成者 (e-Rad)
  • Okita, Akiko K.
  • Zafar, Faria
  • Su, Jie
  • Weerasekara, Dayalini
  • Kajitani, Takuya
  • Takahashi, Tatsuro S.
  • Kimura, Hiroshi
  • Murakami, Yota
  • Masukata, Hisao
  • Nakagawa, Takuro

説明

Heterochromatin that is characterized by histone H3 lysine 9 (H3K9) methylation assembles on repetitive regions including centromeres. Although centromeric heterochromatin is important for faithful segregation of chromosomes, its role in maintaining centromere integrity remains elusive. Here, we found in fission yeast that heterochromatin suppresses gross chromosomal rearrangements (GCRs) at centromeres. Mutations in Clr4/Suv39 methyltransferase increased the formation of isochromosomes whose breakpoints are present in centromere repeats. H3K9A and H3K9R mutations also increased GCRs, suggesting that Clr4 appears to suppress GCRs via H3K9 methylation. Both HP1 homologs, Swi6 and Chp2, and an RNAi component Chp1 were the chromodomain proteins that are essential for full suppression of GCRs. Remarkably, mutations in RNA polymerase II (RNAPII) or the transcription factors including Tfs1/TFIIS which facilitates restart of backtracked RNAPII specifically bypassed the requirement of Clr4 to suppress GCRs. These results demonstrate that heterochromatin suppresses GCRs by repressing Tfs1-dependent transcription of centromere repeats.

Rates of chromosome lossRates of spontaneous chromosome loss were determined using biologically independent cultures.Okita2018_ChL_loss_rate_2.xlsxRates of spontaneous GCRsRates of spontaneous GCRs were determined using biologically independent cultures. A set of GCR rates that appear in one figure in the paper are shown in one sheet.Okita2018_GCR_rate_2.xlsxChromatin Immunoprecipitation (ChIP) dataWe performed ChIP experiments using biologically independent cultures. A set of ChIP data that appear in one figure in the paper are shown in one sheet. The data include the percentage of DNA recovered from ChIP experiments. Each number represents the mean of three measurements in the real time PCR. The value relative to that of adl1 is also shown in one sheet.Okita2018_ChIP_data_2.xlsx

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詳細情報 詳細情報について

  • CRID
    1883398392478056704
  • DOI
    10.5061/dryad.c8n0748
  • 本文言語コード
    en
  • データソース種別
    • OpenAIRE
    • Crossref
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