Peripherally Administered Botulinum Toxin Type a Localizes Bilaterally in Trigeminal Ganglia of Animal Model

DOI IR NDL Digital Collections

Bibliographic Information

Title
Peripherally Administered Botulinum Toxin Type a Localizes Bilaterally in Trigeminal Ganglia of Animal Model
Other Title
  • 片側末梢投与されたA型ボツリヌス毒素は動物モデルにおいて両側三叉神経節に局在する
Author
Waskitho, Arief
Alias Name
  • ワスキソ, アリーフ
  • ワスキソ, アリーフ
Author
Yamamoto, Yumiko
Alias Name
  • ヤマモト, ユミコ
  • ヤマモト, ユミコ
Author
Raman, Swarnalakshmi
Author
Kano, Fumiya
Alias Name
  • 加納, 史也
  • カノウ, フミヤ
Author
Yan, Huijiao
Author
Raju, Resmi
Author
Afroz, Shaista
Author
Morita, Tsuyoshi
Alias Name
  • 守田, 剛
  • モリタ, ツヨシ
Author
Ikutame, Daisuke
Alias Name
  • 生田目, 大介
  • イクタメ, ダイスケ
Author
Okura, Kazuo
Alias Name
  • 大倉, 一夫
  • オオクラ, カズオ
Author
Ohshima, Masamitsu
Alias Name
  • 大島, 正充
  • オオシマ, マサミツ
Author
Yamamoto, Akihito
Alias Name
  • 山本, 朗仁
  • ヤマモト, アキヒト
Author
Baba, Otto
Alias Name
  • 馬場, 麻人
  • ババ, オト
Author
Matsuka, Yoshizo
Alias Name
  • 松香, 芳三
  • マツカ, ヨシゾウ
University
徳島大学
Types of degree
博士（歯学）
Grant ID
甲口第477号
Degree year
2022-03-23

Description

Peripheral nerve injury leads to sensory ganglion hyperexcitation, which increases neurotransmitter release and neuropathic pain. Botulinum toxin type A (BoNT/A) regulates pain transmission by reducing neurotransmitter release, thereby attenuating neuropathic pain. Despite multiple studies on the use of BoNT/A for managing neuropathic pain in the orofacial region, its exact mechanism of transport remains unclear. In this study, we investigated the effects of BoNT/A in managing neuropathic pain in two different animal models and its transport mechanism in the trigeminal nerve. Intraperitoneal administration of cisplatin induced bilateral neuropathic pain in the orofacial region, reducing the head withdrawal threshold to mechanical stimulation. Unilateral infraorbital nerve constriction (IONC) also reduced the ipsilateral head withdrawal threshold to mechanical stimulation. Unilateral peripheral administration of BoNT/A to the rat whisker pad attenuated cisplatin-induced pain behavior bilaterally. Furthermore, contralateral peripheral administration of BoNT/A attenuated neuropathy-induced behavior caused by IONC. We also noted the presence of BoNT/A in the blood using the mouse bioassay. In addition, the Alexa Fluor-488-labeled C-terminal half of the heavy chain of BoNT/A (BoNT/A-Hc) was localized in the neurons of the bilateral trigeminal ganglia following its unilateral administration. These findings suggest that axonal and hematogenous transport are involved in the therapeutic effects of peripherally administered BoNT/A in the orofacial region.

Keywords

Details 詳細情報について

Report a problem

Back to top